Journal: Frontiers in Physiology

Article Title: Integrated proteomics and metabolomics reveal mechanisms of blood pressure reduction in spontaneously hypertensive rats under hypoxic conditions

doi: 10.3389/fphys.2026.1859072

Figure Lengend Snippet: Changes in the protein expression levels of cytochrome c oxidase subunit 5A (Cox5a), NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), ubiquinol-cytochrome c reductase core protein 2 (Uqcrc2), carnitine palmitoyltransferase (Cpt2), acyl-CoA synthetase long chain family member 1 (AcsI1), and kininogen 1 (Kng1) in the abdominal aorta of spontaneously hypertensive rats (SHR) after exposure to high-altitude hypoxic conditions. (A) Western blot images. Corresponding densitometric quantification. Band intensity was normalized to β−actin and quantified using Gel−Pro Analyzer 4 software. (B) Cox5a. (C) Ndufs1. (D) Uqcrc2. (E) Cpt2. (F) Kng1. (G) AcsI1. Data are shown as the mean ± SD (n = 3). SHR-C and SHR-H refer to the SHR control group and SHR high-altitude hypoxia group, respectively. * P < 0.05 vs. SHR-C ( t -test).

Article Snippet: After transfer, membranes were blocked with 5% non-fat milk at room temperature for 2 h, followed by 12–16 h of incubation at 4 °C with primary antibodies against β-actin (1:50000, Abclonal, AC026), AcsI1 (1:2000, Huabio, ER60807), Cox5a (1:2000, Abclonal, A6437), Cpt2 (1:1000, Huabio, ET1611-64), Kng1 (1:1000, Abclonal, A1670), Ndufs1 (1:2000, Abclonal, A21192), and Uqcrc2 (1:2000, Proteinetch, 14742-1-AP).

Techniques: Expressing, Western Blot, Software, Control